Increased In Vitro Lysosomal Function in Oxidative Stress-Induced Cell Lines |
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Authors: | Jihee Yoon Seung Hyuck Bang Jin-Soo Park Suk-Tai Chang Yang-Hoon Kim Jiho Min |
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Institution: | (1) Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, 664-14 Deokjin-dong, 1Ga Deokjin-Gu, Jeonju, 561-756, South Korea;(2) Department of Bioprocess Engineering, Chonbuk National University, 664-14 Deokjin-dong, 1Ga Deokjin-Gu, Jeonju, 561-756, South Korea;(3) Department of Environmental Engineering, College of Engineering, Sangmyung University, 300 Anseo-dong, Dongnam-gu, Cheonan, Chungnam Province, 330-720, South Korea;(4) School of Chemical Engineering and Materials Science, Chung-Ang University, 221 Heukseok-dong, Dongjak-gu, Seoul, 156-756, South Korea;(5) Department of Microbiology, Chungbuk National University, 410 Sungbong-Ro, Heungduk-Gu, Cheongju, 361-763, South Korea; |
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Abstract: | Exposure of mammalian cells to oxidative stress alters lysosomal enzymes. Through cytochemical analysis of lysosomes with
LysoTracker, we demonstrated that the number and fluorescent intensity of lysosome-like organelles in HeLa cells increased
with exposure to hydrogen peroxide (H2O2), 6-hydroxydopamine (6-OHDA), and UVB irradiation. The lysosomes isolated from HeLa cells exposed to three oxidative stressors
showed the enhanced antimicrobial activity against Escherichia coli. Further, when lysosomes that were isolated from HeLa cells exposed by oxidative stress were treated to normal HeLa cells,
the viability of the HeLa cells was drastically reduced, suggesting increased in vitro lysosomal function (i.e., antimicrobial
activity, apoptotic cell death). In addition, we also found that cathepsin B and D were implicated in increased in vitro lysosomal
function when isolated from HeLa cells exposed by oxidative stress. Decrease in cathepsin B activity and increase in cathepsin
D activity were observed in lysosomes isolated from HeLa cells after treatment with H2O2, 6-ODHA, or UVB, but cathepsin B and D were not the sole factors to induce cell death by in vitro lysosomal function. Therefore,
these studies suggest a new approach to use lysosomes as antimicrobial agents and as new materials for treating cancer cell
lines. |
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