Evaluation of Glycosyl Hydrolases in the Secretome of <Emphasis Type="Italic">Aspergillus fumigatus</Emphasis> and Saccharification of Alkali-Treated Rice Straw |
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Authors: | Manju Sharma Rohit Soni Asiya Nazir Harinder Singh Oberoi Bhupinder Singh Chadha |
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Institution: | (1) Department of Microbiology, Guru Nanak Dev University, Amritsar, 143005, Punjab, India;(2) Central Institute of Post Harvesting Technology, Ludhiana, 141004, Punjab, India |
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Abstract: | A thermotolerant Aspergillus fumigatus strain isolated from composting pile of mixed industrial waste was found to produce a spectrum of cellulase and hemicellulases
when cultured on rice straw solidified substrate. The two-dimensional electrophoresis (2DE) resolved the secretome into 57
distinct protein spots. The zymograms developed against 2DE gels identified the presence of three β-glucosidases and five
CBHI/EGI isoforms in the secretome. The peptide mass fingerprinting of 17 protein spots by liquid chromatography mass spectrometry
characterized the secretome into different glycosyl hydrolase families. The enzyme cocktail produced by A. fumigatus was capable of efficient hydrolysis of alkali pretreated rice straw (at 7% and 10% w/v) resulting in 95% and 91% saccharification, respectively. |
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