Decolorization of Naphthol Blue Black using the Horseradish Peroxidase |
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| Authors: | Selva Onder Mithat Celebi Melda Altikatoglu Arzu Hatipoglu Huriye Kuzu |
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| Institution: | (1) Faculty of Arts and Sciences, Department of Chemistry, Yildiz Technical University, Davutpasa Campus 34210 Esenler, Istanbul, Turkey;(2) Faculty of Engineering, Department of Polymer Engineering, Yalova University, 77100 Yalova, Turkey;(3) Faculty of Chemical and Metallurgical Engineering, Department of Bioengineering, Yildiz Technical University, Davutpasa Campus 34210 Esenler, Istanbul, Turkey; |
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| Abstract: | This study evaluates the potential of the enzyme horseradish peroxidase in the decolorization of one common industrial azo
dye, naphthol blue black. Studies are carried out to understand the process parameters such as pH, temperature and reaction
time. The enzymatic decolorization of the dye was examined by UV-Vis spectrophotometer and LC-MS measurements. Temperature
and pH conditions were optimized for obtaining high azo-dye decolorization. Azo-dye removal at a pH range 4-6 was found to
be the highest for all temperatures. After 5 minutes of treatment, the color removal of dye was ca. 80-90%. The LC-MS and
spectrophotometric analyses indicated that the decolorization of the azo dye with enzyme was due to the reduction of the azo
bonds. This study verifies the viability of the use of the horseradish peroxidase in the decolorization of naphthol blue black. |
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