结直肠癌患者血清FTIR光谱的初步研究

对31例结直肠癌患者、8例肠炎患者和10例健康者的血清冻干粉标本进行傅里叶变换红外光谱(fourier transformation infrared spectrum, FTIR)检测，对比分析各组红外光谱特征。结果显示，FTIR检测血清冻干粉获得11个吸收峰，结直肠癌组P9峰位波数蓝移(1 249 cm-1)，差异有统计学意义(Z=-2.051, p<0.05);5个吸光度比值组间差异无统计学意义;层序聚类分析法(hierarchical cluster analysis，HCA)显示结直肠癌个体红外光谱具有一定程度的聚类特性;对酰胺Ⅰ带光谱(1 700～1 600 cm-1)进行傅里叶解卷积等处理获得蛋白二级结构的百分含量，组间差异无统计学意义。研究结果初步提供了血清FTIR光谱用于结直肠癌早期诊断的理论依据。

Initial Research on FTIR Spectroscopy of Lyophilized Serum from Colorectal Cancer Patients

In the present paper, lyophilized sera from 31 patients with colorectal cancer，8 patients with enteritis and 10 healthy controls were measured using Nicolet Nexus-670 Fourier Transform Infrared (FTIR) spectrometer, and the spectral features were investigated and comparison among three groups was made. The result showed that (1) there are 11 peaks of absorption in the spectrum of lyophilized sera, the wavenumber of the ninth peak moved to 1 249 cm-1 in the colorectal cancer group and significant difference was observed with the control group (Z=-2.051, P<0.05); Five ratios of absorbance were calculated and no significant difference was observed among three groups; (2) spectrum in the region of 1 700～1 000 cm-1 was analysed with the method of hierarchical cluster analysis and showed only partial success in distinguishing the three groups; (2) spectrum of amide Ⅰ peptide (1 700～1 600 cm-1) was deconvolved to get the percentage composition of protein secondary structures, and no significant difference was observed among three groups. The results provide a preliminary theoretical basic for using FTIR spectrum of lyophilized serum for the early diagnosis of colorectal cancer.