| 高效液相色谱法同时测定假尿核苷和肌酐的研究及应用 |
| |
| 引用本文： | 许丹科,谭力.高效液相色谱法同时测定假尿核苷和肌酐的研究及应用[J].分析化学,1993,21(9):993-995. |
| |
| 作者姓名： | 许丹科 谭力 |
| |
| 作者单位： | 南京军区南京总医院全军医学检验中心，南京军区南京总医院全军医学检验中心，南京军区南京总医院全军医学检验中心 南京 210002，南京 210002，南京 210002 |
| |
| 摘 要： | 本文用高效液相色谱法同时测定人尿中假尿核苷和肌酐,研究了流动相的pH值变化对色谱行为的影响,方法简便、快速,已成功地用于对正常人、肺部感染及肺癌患者的尿样分析,其结果有显著性差异。
|
| 关 键 词： | 假尿核苷 肌酐 高效液相色谱 尿 |
Simultaneous Determination of Pseudouridine andCreatinine by High-performance Liquid Chromatographyand Its Analytical Application |
| |
| Xu Danke,Tan Li,Yuan Yisheng.Simultaneous Determination of Pseudouridine andCreatinine by High-performance Liquid Chromatographyand Its Analytical Application[J].Chinese Journal of Analytical Chemistry,1993,21(9):993-995. |
| |
| Authors: | Xu Danke Tan Li Yuan Yisheng |
| |
| Abstract: | A simple method for the simultaneous determination of pseudouridine and creatinine in urine using reversed-phase high-performance liquid chromatography with ultraviolet detection is described. It consists of simply diluting the filtered urine with mobile phase followed by direct chromatographic injection. The mobile phase was 0. 02mol/L phosphate buffer (pH=4. 0) and wavelength of detection was at 254nm. Influences of pH of the mobile phase on capacity factors have also been discussed. This method has been applied to the analysis of urine samples from normal donors and patients with lung cancer and pulmonary infectious. |
| |
| Keywords: | Pseudouridine Creatinine High-performance liquid chromatography |
| 本文献已被 CNKI 维普 等数据库收录！ |
