Natural Compounds against Neurodegenerative Diseases: Molecular Characterization of the Interaction of Catechins from Green Tea with Aβ1–42, PrP106–126, and Ataxin‐3 Oligomers |
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| Authors: | Dr. Erika Sironi Dr. Laura Colombo Angela Lompo Dr. Massimo Messa Dr. Marcella Bonanomi Dr. Maria Elena Regonesi Dr. Mario Salmona Dr. Cristina Airoldi |
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| Affiliation: | 1. Department of Biotechnology and Biosciences University of Milano‐Bicocca, P.zza della Scienza, 2, 20126, Milano (Italy), Fax: (+39)?02‐6448‐3565;2. Department Biochemistry and Molecular Pharmacology, IRCCS‐Istituto di Ricerche Farmacologiche “Mario Negri”, Via Giuseppe La Masa, 19 20156 Milano (Italy);3. Department of Statistics and Quantitative Methods, University of Milano‐Bicocca, Via Bicocca degli Arcimboldi, 8, 20126, Milano (Italy) |
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| Abstract: | By combining NMR spectroscopy, transmission electron microscopy, and circular dichroism we have identified the structural determinants involved in the interaction of green tea catechins with Aβ1–42, PrP106–126, and ataxin‐3 oligomers. The data allow the elucidation of their mechanism of action, showing that the flavan‐3‐ol unit of catechins is essential for interaction. At the same time, the gallate moiety, when present, seems to increase the affinity for the target proteins. These results provide important information for the rational design of new compounds with anti‐amyloidogenic activity and/or molecular tools for the specific targeting of amyloid aggregates in vivo. |
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| Keywords: | amyloid peptides circular dichroism molecular recognition structure– activity relationship NMR spectroscopy |
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