Separation of the enantiomers of tebuconazole and its potential impurities by high-performance liquid chromatography with a cellulose derivative-based chiral stationary phase

Summary The high-performance liquid chromatographic resolution of the enantiomers of tebuconazole, a new anti-fungal agent with one chiral center, and the enantiomers of some impurities in technical tebuconazole, has been studied on a chiral stationary phase prepared by coating aminopropylated silica gel with celluloseris(3,5-dimethylphenylcarbamate). The effects of solute structure and the amount of the organic mobile-phase modifier, 2-propanol, on retention and resolution were studied. Under optimum conditions excellent enantiomer separations were achieved for tebuconazole and its impurities. As far as we are aware this is the only liquid chromatographic system enabling discrimination of the enantiomers of all of the racemates discussed in this paper.