Efficiency of a fixed-bed and a gas-lift three-column reactor for continuous production of ethanol by pectate-and alginate-immobilized Saccharomyces cerevisiae cells |
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| Authors: | M Valach M Navrátil V Horváthová J Zigová E Šturdík E Hrabárová P Gemeiner |
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| Institution: | (1) Department of Biochemical Technology, Faculty of Chemical and Food Technology, Slovak University of Technology, SK-812 37 Bratislava, Slovakia;(2) Department of Biotechnology, Faculty of Natural Sciences, University of St. Cyril and Methodius, SK-917 01 Trnava, Slovakia;(3) Institute of Chemistry, Slovak Academy of Sciences, SK-845 38 Bratislava, Slovakia |
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| Abstract: | Ethanol-tolerant and thermo-tolerant yeast strain Saccharomyces cerevisiae C11-3 cells immobilized in calcium pectate and calcium alginate gels were used for ethanol fermentation in a three-reactor
system with a gradient temperature control. The fermentation process has been tested in a fixed-bed and a gas-lift arrangement.
The gas-lift system was more efficient due to a better mass transport between the phases. Abrasion was more evident in calcium
alginate particles, while calcium pectate beads were not significantly damaged. Two different concentrations of alginate were
tested and calcium pectate gel was demonstrated to be more suitable as an immobilization material in comparison with calcium
alginate due to its mechanical resistance and favourable diffusion parameters, providing an ethanol production of more than
7.5 g dm−3 h−1 over a period of 630 h. |
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