Automated Solid-Phase Extraction and Liquid Chromatography Tandem Mass Spectrometry Determination of N-methyl-2-pyrrolidone and its Metabolites in Urine and Plasma |
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Authors: | A S Cohen B A G Jönsson |
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Institution: | (1) Department of Occupational and Environmental Medicine, Lund University Hospital, Lund, 22185, Sweden |
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Abstract: | A method for the simultaneous determination of N-methyl-2-pyrrolidone (NMP) and its metabolites 5-hydroxyl-N-pyrrolidone (5HNMP), N-methylsuccinimide (MSI) and 2-hydroxy-N-methylsuccinimide (2HMSI) in plasma and urine has been developed. Samples were purified by SPE using an ASPEC XL4. Analysis
was performed using LC–MS equipped with an APCI interface. The analysis provided linear responses in the range of 0.125–12 μg
mL−1 for all of the analytes and up to 150 μg mL−1 for 5HNMP and 2HMSI. The within day precision was in the range of 0.9–19.1% for plasma samples and 1.9–10.4% for urine samples
whereas the between day precisions were 4.5–11.9% and 1.2–17.5%, respectively. The method was deemed to be suitable for monitoring
the levels of NMP and its metabolites in the plasma and urine of occupationally exposed persons. |
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Keywords: | Column liquid chromatography– mass spectrometry Solid-phase extraction N-methyl-2-pyrrolidone in Urine and plasma |
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