Glucose Biosensor Based on the Fabrication of Glucose Oxidase in the Bio-Inspired Polydopamine-Gold Nanoparticle Composite Film

A highly efficient enzyme immobilization method has been developed for electrochemical biosensors using polydopamine films with gold nanoparticles (AuNPs) embedded. This simple enzyme fabrication method can be performed in very mild conditions and stored in a long time with high bioactivity. The fabricated amperometric glucose biosensor exhibited a high and reproducible sensitivity, wide linear dynamic range and low limit of detection (LOD) (0.1 μmol·L^?1). A low value of 1.5 mmol·L^?1 for the apparent Michaelis‐Menten constant K^app_M was obtained. The high sensitivity, wide linear range, good reproducibility and stability make this biosensor a promising candidate for portable amperometric glucose biosensor.