Pulsed optoacoustic spectroscopy for calorimetry wihout a standard |
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| Affiliation: | 1. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan, 030006, China;2. Key Laboratory of Functional Molecules of Shanxi Province, Research Institute of Applied Chemistry, Shanxi University, Taiyuan, 030006, China;3. Second Hospital of Shanxi Medical University, Taiyuan, 030001, China;1. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China;2. Key Laboratory of Functional Molecules of Shanxi Province, Research Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China;3. Institute of Resources and Environment Engineering, Shanxi University, Taiyuan 030006, China;1. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan, 030006, China;2. Research Institute of Applied Chemistry, Shanxi University, Taiyuan, 030006, China;3. Xinzhou Teachers University, Xinzhou, 030004, China |
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| Abstract: | A calorimetric method is described which does not require a reference absorber to deduce absolute yields for heat deposition following pulsed laser irradiation. Pulsed optoacoustic detection is used and signals are calibrated based on the excitation wavelength dependence of the heat yield. The apparatus is used to measure fluorescence yields of several well known dyes in methanol solution. It is also demonstrated on a monolayer of dye adsorbed to sapphire where no reference would be available. This technique was originally developed to measure pulsed temperature rises in stained mitochondria, and to choose an appropriate staining dye for selective thermal denaturation of proteins in cancerous cells by pulsed laser heating. |
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