Ratiometric fluorescence detection of a tag fused protein using the dual-emission artificial molecular probe |
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| Authors: | Honda Kei Nakata Eiji Ojida Akio Hamachi Itaru |
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| Affiliation: | Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Nishikyo-ku, Kyoto, 615-8510, Japan. |
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| Abstract: | We have successfully developed a ratiometric detection system for protein of interest using the complementary recognition pair of the tetra-aspartate peptide tag and the SNARF-appended Zn(ii)-DpaTyr probe. |
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