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In vitro POLLEN PLANT INDUCTION AND EMBRYOID CLONE ESTABLISHMENT OF Panax ginseng
作者姓名:杜令阁  侯艳华  常维春  李安生  傅志明  邵启全  陈泽光  杨振棠
作者单位:Institute of Special Local Products,CAAS,Jilin,Institute of Special Local Products,CAAS,Jilin,Institute of Special Local Products,CAAS,Jilin,Institute of Genetics,Academia Sinica,Beijing,Institute of Genetics,Academia Sinica,Beijing,Institute of Genetics,Academia Sinica,Beijing,Jilin City Institute of Agricultural Sciences,Jilin City Institute of Agricultural Sciences
摘    要:In anther culture of Panax ginseng, its callus formation showed a wide adaptability to culture media. Large numbers of calli were induced on media exhibiting better effects of induction. Supplemeres of 5 mg 2,4-D/1 and 1 mg KT/1 to the media proved to be much effective. Regeneration of the whole plantlets from anther culture of Panax ginseng is usually quite difficult. During the past three years, however, sixteen of the 100 medium formulae tested were proved to be suitable. The formulae of MS+0.5 mg BA/1+2 mg GA/1+1000 mg LH/1+3% sucrose were considered good and effective. A visibly differentiated body, which was light-milky white and later turned into a light green spot, was formed 40 days after the callus was transferred to the differentiation media. This body differentiated subsequently into buds, roots and, eventually, seedlings. The embryoid clones have been established in order to maintain its ability of continual differentiation into plantlets through successive culturings of many generations.

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