|
|||||
|
|
Papain incorporated chitin dressings for wound debridement sterilized by gamma radiation |
|
| Authors: | Durgeshwer Singh Rita Singh |
| Institution: | 1. Department of Food Science, College of Agriculture, Isfahan University of Technology, Isfahan 84156, Iran;2. Department of Chemistry, Catalysis Division, University of Isfahan, Isfahan 81746-73441, Iran;1. Laboratory of Ethnobotany and Natural Substances, Department of Natural Sciences, Ecole Normale Supérieure (ENS), Kouba, Algiers, Algeria;2. Faculty of Sciences, Natural and Life Sciences Department, Mohamed Boudiaf University, M’sila, Algeria;3. School of Pharmacy, University of Camerino, Camerino, Italy;1. CSIR-Central Leather Research Institute, Chennai 600 020, Tamil Nadu, India;2. Department of Virology, King Institute of Preventive Medicine and Research, Chennai 600 032, Tamil Nadu, India;1. Department of Surgery, Haeundae Paik Hospital, College of Medicine, Inje University, Busan, Republic of Korea;2. Department of Surgery, College of Medicine, Chung-Ang University Hospital, Seoul, Republic of Korea;3. Department of Surgery, Catholic Kwandong University, International St. Mary''s Hospital, Incheon, Republic of Korea;4. Department of Anesthesiology, Haeundae Paik Hospital, College of Medicine, Inje University, Busan, Republic of Korea |
| Abstract: | Wound debridement is essential for the removal of necrotic or nonviable tissue from the wound surface to create an environment conducive to healing. Nonsurgical enzymatic debridement is an attractive method due to its effectiveness and ease of use. Papain is a proteolytic enzyme derived from the fruit of Carica papaya and is capable of breaking down a variety of necrotic tissue substrates. The present study was focused on the use of gamma radiation for sterilization of papain dressing with wound debriding activity. Membranes with papain were prepared using 0.5% chitin in lithium chloride/dimethylacetamide solvent and sterilized by gamma radiation. Fluid absorption capacity of chitin–papain membranes without glycerol was 14.30±6.57% in 6 h. Incorporation of glycerol resulted in significant (p<0.001) increase in the absorption capacity. Moisture vapour transmission rate of the membranes was 4285.77±455.61 g/m2/24 h at 24 h. Gamma irradiation at 25 kGy was found suitable for sterilization of the dressings. Infrared (IR) spectral scanning has shown that papain was stable on gamma irradiation at 25–35 kGy. The irradiated chitin–papain membranes were impermeable to different bacterial strains and also exhibited strong bactericidal action against both Gram-positive and Gram-negative bacteria. The fluid handling characteristics and the antimicrobial properties of chitin–papain membranes sterilized by gamma radiation were found suitable for use as wound dressing with debriding activity. |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ | |