Synthesis and spectroscopic studies of model red fluorescent protein chromophores |
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Authors: | He Xiang Bell Alasdair F Tonge Peter J |
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Institution: | Department of Chemistry, State University of New York at Stony Brook, Stony Brook, New York 11794-3400, USA. |
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Abstract: | reaction: see text]. Here we describe the synthesis and spectroscopic characterization of two compounds designed to model the chromophore in DsRed, a red fluorescent protein. Comparison with model green fluorescent protein (GFP) chromophores indicates that the additional conjugation in the DsRed models can account, in part, for the red-shifted absorption and emission properties of DsRed compared to those of GFP. In contrast to the GFP models, the DsRed models are fluorescent with quantum yields of 0.002-0.01 in CHCl3. |
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