| 三试剂自动分析法测定血清总铁结合力：Nitro—PAPS直接光度法 |
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| 引用本文： | 谢作叙,连国军,赵长容,曹建明. 三试剂自动分析法测定血清总铁结合力：Nitro—PAPS直接光度法[J]. 广东微量元素科学, 2008, 15(9): 30-33 |
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| 作者姓名： | 谢作叙 连国军 赵长容 曹建明 |
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| 作者单位： | 温州医学院环境与公共卫生学院,浙江,温州,325003 |
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| 基金项目： | 浙江省温州市科技计划，浙江省教育厅资助项目 |
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| 摘 要： | 为建立简便、灵敏的三试剂自动化分析法测定血清总铁结合力（TIBC），在表面活性剂存在下，用2-（5-硝基-2-吡啶偶氮）-5-[N-正丙基-N-（3-磺酸丙基）氨基]苯酚二钠（Nitro—PAPS）为显色剂测定了血清TIBC。结果表明，该法线性范围0～195μmol／L，平均回收率为100．7％，批内变异系数（CV）和批间变异系数分别为0．019、0．032；与临床检验操作规程推荐的方法比较具有良好的相关性，线性回归方程和相关系数分别为Y=1．0021x＋1．2100，r=0．9798；78例健康男性血清TIBC含量（x±2s）为46．8—76．1μmol／L，75例健康女性为52．8—77．6μmol／L。方法简便、灵敏可靠，适合临床应用。
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| 关 键 词： | 总铁结合力 血清 自动分析法 Nitro—PAPS |
A New Automatic Method for Analysis of TIBC in Serum with Three Reagents:Nitro-PAPS Direct Spectrophotometric Method |
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| XIE Zuoxu,LIAN Guojun,ZHAO Changrong,CAO Jianming. A New Automatic Method for Analysis of TIBC in Serum with Three Reagents:Nitro-PAPS Direct Spectrophotometric Method[J]. Trace Elements Science, 2008, 15(9): 30-33 |
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| Authors: | XIE Zuoxu LIAN Guojun ZHAO Changrong CAO Jianming |
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| Affiliation: | XIE Zuoxu,LIAN Guojun,ZHAO Changrong,CAO Jianming.(School of Public Health,Wenzhou Medical College,Wenzhou 325000,China) |
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| Abstract: | To establish a simple, sensitive automatic method of determination of TIBC in serum with three reagents, serum TIBC was determined with Nitro - PAPS in the presence of surfactant. The results showed that the method was liner in the range of 0 - 195 μmol/L, the average rate of recovery was 100. 7%, the withinrun and between run CV was 0. 019 and 0. 032, respectively. Comparing this method （y） with refence method （x）, the regression equation was obtained y = 1. 002 1 x ＋ 1. 210 0, r = 0. 979 8. The reference interval （x ＋ 2s） was 46. 8 - 76. 1 μmol/L for male （ n = 78） , and 46.8 76. 1 μmoL/L for female （n = 75 ）. It concludes this method is simple, sensitive and suitable for clinical application. |
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| Keywords: | Nitro-PAPS |
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