Synchronous fluorescence determination of ciprofloxacin in the pharmaceutical formulation and human serum based on the perturbed luminescence of rare-earth ions |
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Authors: | Changlun Tong Xiajun Zhuo Yun Guo Yueheng Fang |
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Affiliation: | Key Laboratory of Environmental Remediation and Ecological Health, Ministry of Education, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310029, PR China |
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Abstract: | A simple, rapid and sensitive synchronous fluorescence method was developed for the determination of ciprofloxacin (CPFX) in the pharmaceutical formulation and human serum. The results show that when Y3+ is added into the CPFX solution, the characteristic fluorescence of Y3+ is not emitted whereas the fluorescence intensity of CPFX is significantly enhanced. The synchronous fluorescence technology is employed in this method to directly determine trace amount of CPFX in human serum. A linear relationship between the fluorescence intensity and the CPFX concentration is obtained in the range of 1.0×10−9 ∼5.0×10−6 mol L−1. The limit of detection (LOD) of this method attains as low as 2.0×10−10 mol L−1 (S/N=3). The selectivity of this method is also very good. Common metal ions, rare-earth ions and some pharmaceuticals, which are usually used together with CPFX in the clinic, do not interfere with the determination of CPFX under general conditions. |
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Keywords: | Ciprofloxacin Fluoroquinolones Synchronous fluorescence spectrometry Yttrium (III) Human serum |
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