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Time variation of fluorescence lifetime in enhanced cyan fluorescence protein |
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| Authors: | Soonhyouk Lee Kyoungsook Park Bong Hyun Chung Sok Won Kim |
| Affiliation: | a Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon, Korea b BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, aejeon, Korea c University of Science and Technology, Daejeon, Korea d Department of Physics, University of Ulsan, Ulsan, Korea |
| Abstract: | The lifetime variations of enhanced cyan fluorescence protein (ECFP) in relatively short integration time bins were studied via time-correlated single photon counting (TCSPC) measurement. We observed that minimum photon counts are necessary for the lifetime estimation to achieve a certain range of variance. The conditions to decrease the variance of lifetime were investigated and the channel width of the measurement of TCSPC data was found to be another important factor for the variance of lifetime. Though the lifetime of ECFP is best fit by a double exponential, a mono exponential fit for the same integration time is more stable. The results may be useful in the analysis of photophysical dynamics for ensemble molecules in short measurement time windows. |
| Keywords: | TCSPC Lifetime ECFP |
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