Thermochemistry of the reaction catalyzed by lactate dehydrogenase |
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| Authors: | Larry Donnovan Kathryn Barclay Kenneth Otto Neil Jespersen |
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| Affiliation: | Department of Chemistry, The University of Texas at Austin, Austin, Texas 78712 U.S.A. |
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| Abstract: | The thermochemistry of the reduction of pyruvate to lactate, in the presence of nicotinamide adenine dinucleotide (reduced form); catalyzed by the enzyme lactate dehydrogenase, has been studied. After approximately 120 experiments, a best value for the enthalpy of reaction has been determined to be ?14.80±0.30 kcal mol?1. This reveals that the driving force for the reaction is almost completely enthalpic in nature. In addition, using the current methodology, it is possible to determine lactate dehydrogenase activity as low as 0.15 international units (325 Wroblewski units) per sample. |
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| Keywords: | To whom requests for reprints should be sent. |
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