Optimization of fat soluble vitamin separation in reversed-phase microcolumn liquid chromatography |
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| Authors: | W. T. Wahyuni K. Jinno |
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| Affiliation: | (1) School of Materials Science, Toyohashi University of Technology, 440 Toyohashi, Japan |
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| Abstract: | Summary Separation behavior of fat soluble vitamins on various chemically bonded materials in non-aqueous reversedphases microcolumn liquid chromatography has been investigated. The best performance was found on a polymer-based octadecyl bonded phase. Optimization of separation for vitamins D2, D3, E and E acetate has been tried using this polymer-based column and it has been found that a binary mobile phase consisting of acetonitrile and methanol gave complete separation of fat soluble vitamins. |
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| Keywords: | Column liquid chromatography Microbore capillary columns Polymer based octadecylsilica bonded phase Fat soluble vitamins |
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