酮洛芬分子印迹拆分及分离过程热力学研究

利用非共价分子印迹法，采用4-乙烯基吡啶为功能单体，以二甲基丙烯酸乙二 醇酯为交联剂，在模板分子（S）-酮洛芬的存在下，制备出（S）-酮洛芬的分子印 迹聚合物（MIPs），并用作HPLC固定相，对其进行了高效液相色谱评价。结果表明 外消旋酮洛芬在制备的印迹柱上得到了有效的分离，（S）-酮洛芬的容量因子 k_s'为9.52，选择性因子α为1.52，分离度R为0.88。此外，HPLC分析表明制备的 MIPs能够分离结构相似的酮洛芬、布洛芬和萘普生。研究了流动相中乙酸浓度、流 速及温度对拆分效果的影响，测定了分离过程中的焓变、熵变和自由能变化，对分 子印迹分离过程作了进一步解释。

Chiral Separation of Ketoprofen and Thermodynamic Study in Molecular Imprinting

A chiral stationary phase for the enantioselective separation of ketoprofen has been prepared by molecular imprinting, in which 4- vinylpyridine (functional monomer) and ethylene glycol dimethacrylate were copolymerised in the presence of (S)-ketoprofen. Racemic ketoprofen was efficiently resolved on the molecular imprinting polymer. Capacity factor (k_s') of (S)-ketoprofen and separation factor (α) were 9.52 and 1.52, respectively. Furthermore, the polymer was able to separate ketoprofen from the structurally related ibuprofen and naproxen. Effects of concentration of acetic acid in the mobile phase, flow rate and temperature were studied. The change of enthalpy and entropy were estimated. It was found that enantioselective separation was enthalpy controlled.