Simultaneous Determination of Nine Flavonoids in Dalbergia odorifera by LC |
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Authors: | Rong-Xia Liu Li Li Qiao Wang Wei Wang Kai-Shun Bi De-An Guo |
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Institution: | (1) School of Pharmaceutical Sciences, Peking University, Beijing, 100083, P. R. China;(2) Shenyang Pharmaceutical University, Shenyang, 110016, P. R. China |
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Abstract: | A rapid and accurate HPLC method has been developed for the simultaneous determination of nine major flavonoids, namely 3 -hydroxymelanettin, melanettin, stevenin, butein, isoliquiritigenin, dalbergin, 2,4-dihydroxy-5-methoxybenzophenone, pinocembrin, 4-methoxydalbergione in Dalbergia odorifera. The samples were separated on an Aglient Zorbax SB-C18 column (250 × 4.6 mm, 5 m) with a gradient of acetonitrile and 1% aqueous acetic acid ( / ) at a flow rate of 1.0 mL min–1 and detected at 350 nm. The complete separation was obtained within 30 min for the nine target flavonoids. All calibration curves showed good linearity (r2 > 0.999) within test ranges. The repeatability was evaluated by intra- and inter-day assays and RSD values were less than 4.0%. The recoveries were between 92.0% and 104.5%. The developed method was successfully applied to the analysis of 32 commercial samples of D. odorifera. |
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Keywords: | Column liquid chromatography Flavonoids Dalbergia odorifera |
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