A 2D NMR method to study peptide phosphorylation |
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Authors: | Christian Raeck Stefan Berger |
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Affiliation: | (1) Institute of Analytical Chemistry, University of Leipzig, Linnéstr. 3, Leipzig, 04103, Germany |
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Abstract: | We demonstrate a 2D NMR method which distinguishes between phosphorylated and non-phosphorylated amino acids. The method is capable of monitoring the amino acid and site-specific enzymatic phosphorylation and dephosphorylation of peptides. The method was developed using O-phosphorylated amino acids and its potential is shown with a peptide fragment of the myelin basic protein (MBP). |
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Keywords: | Phosphorylation NMR Peptides Kinase Phosphatase |
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