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| Fusion Proteins Cpn10-E^rns with Properties of Generating CSFV-Neutralized Antibodies | |
| 作者姓名： | CHEN Yue WANG Xuan-jun CONG Xian-ling ZHANG Pei-yin WU Xiu-li WEI Hong-fei WANG Li WAN Min WANG Li-ying YU Yong-li |
| 作者单位： | CHEN Yue~ 1，3，WANG Xuan-jun~1，CONG Xian-ling~ 1，4，ZHANG Pei-yin~2，WU Xiu-li~2，WEI Hong-fei~2，WANG Li~1，WAN Min~ 2，WANG Li-ying~ 2** and YU Yong-li~ 2** 1. Department of Immunology，2. Department of Molecular Biology，3. College of Life Science，4. China-Japan Union Hospital，Jilin University，Changchun 130021，P.R. China |
| 基金项目： | 国家重点基础研究发展计划(973计划) |
| 摘 要： | IntroductionClassical swine fever(CSF), included in the listA of the Office International des Epizooties(OIE), is ahighly contagious disease of domestic pigs and is con-sidered as the mostharmful disease occurring in pigs allover the world1]. Outbreaks o… |
| 关 键 词： | 经典猪瘟病毒 E^rns 疫苗 熔解蛋白 |
| 文章编号： | 1005-9040（2006）-06-712-05 |
| 收稿时间： | 2006-03-21 |
Fusion Proteins Cpn10-E with Properties of Generating CSFV-Neutralized Antibodies |
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| CHEN Yue WANG Xuan-jun CONG Xian-ling ZHANG Pei-yin WU Xiu-li WEI Hong-fei WANG Li WAN Min WANG Li-ying YU Yong-li.Fusion Proteins Cpn10-E^rns with Properties of Generating CSFV-Neutralized Antibodies[J].Chemical Research in Chinese University,2006,22(6):712-716. | |
| Authors: | Yue CHEN Xuan-jun WANG Xian-ling CONG Pei-yin ZHANG Xiu-li WU Hong-fei WEI Li WANG Min WAN Li-ying WANG Yong-li YU |
| Institution: | aDepartment of Immunology bDepartment of Molecular Biology cCollege of Life Science dChina-Japan Union Hospital, Jilin University, Changchun 130021, P. R. China |
| Abstract: | When pigs are infected with classical swine fever virus (CSFV), the antibody primarily targets the structural glycoprotein E rns of the virus. Previous investigations have demonstrated that E rns has low or no virus neutralizing capacity. In this study, candidate subunit marker vaccine, chaperonin 10(Cpn10)-Erns, which possess the property of generating neutralized antibodies against lethal challenge of virulent CSFV was developed. The gene of E rns was isolated from Hog cholera lapinized virus (HCLV)-infected spleen cells of rabbits via RT-PCR method and fused to the downstream region of the cpn10 gene; the products of recombinant fusion protein (cpn10-Erns) induced expression in Escherichia coli, and the products were purified by affinity chromatography. During the course of vaccination, the candidate vaccines cpn10-E rns were used for the immunization of guinea pigs, and they induced a strong antibody response against cpn10-Erns. The antibodies can be immobilized by coating inactivated CSFV particles, indicating that these antibodies can recognize CSFV. Neutralization assay was carried out on rabbits according to National Regulations on Veterinary Drug. The results clearly indicate that the typical fever of rabbits induced by the live attenuated HCLV could be inhibited by preincubation with the antisera (dilution 1:4) induced by cpn10-Erns, but not inhibited by preincubation with the antisera induced only by Erns. Analogous results were observed for the group of the rabbits immunized with cpn10-Erns, which were protected against the typical fever induced by the challenge with HCLV. The findings of this study formed the basis of a new means for developing subunit marker vaccine against CSFV. |
| Keywords: | Classical swine fever virus E~ rns Chaperonin Marker vaccine |
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