Electrophoretic isolation of membrane proteins from acrylamide gels |
| |
Authors: | Hermann Schägger |
| |
Institution: | 1. Zentrum der Biologischen Chemie, Universit?tsklinikum Frankfurt, Theodor-Stern-Kai 7, Haus 25B, 60590, Frankfurt am Main, Germany
|
| |
Abstract: | Protocols for the optimal resolution of membrane and watersoluble proteins in SDS-denatured state (Tricine SDS-PAGE and Blue
Tricine SDS-PAGE; Laemmli SDS-PAGE and Blue Laemmli SDSPAGE) and in the native state (Blue Native PAGE) are presented. The
protocols for protein recovery from these gels include techniques of electroelution and electroblotting optimized to the type
of the preceding electrophoresis system. Native and denatured proteins thus are obtainable in near quantitative yield in soluble
and in immobilized form. These techniques can optionally be performed in the milligram range, e.g., for the use of immunization
and N-terminal protein sequencing, or in the analytical range. |
| |
Keywords: | Blue Native Electrophoresis Blue SDS Electrophoresis membrane proteins electroelution electroblotting |
本文献已被 SpringerLink 等数据库收录! |
|