Characterization ofC-glycosylflavones fromDissotis rotundifolia by liquid chromatography — UV diode array detection — tandem mass spectrometry |
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Authors: | G Rath A Touré M Nianga J L Wolfender K Hostettmann |
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Institution: | (1) Institut de Pharmacognosie et Phytochimie, Université de Lausanne, BEP, 1015, Lausanne, Switzerland;(2) Departement de Pharmacie, Faculté de Médecine, Université de Conakry, Conakry, République de Guinée;(3) Laboratoire des Composés Naturels (LACONA), B. P. 561, Conakry, République de Guinée |
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Abstract: | Summary By high-performance liquid chromatography (HPLC) coupled with UV diode-array detection (DAD) and thermospray mass spectrometry
(TSP-MS), four main constituents of a polar, whole plant extract fromDissotis rotundifolia T. were characterized. The fourC-glycosylflavones, isoorientin, orientin, vitexin and isovitexin were detected in the methanolic and hydroalcoholic extract
of the plant as well as in the commercial drug preparation ‘Sirop de Dissotis’. Although the UV data and TSP mass spectra
allowed rapid characterisation of all fourC-glycosylflavones, exact attribution of the peaks to their structures could not be achieved as neither the UV spectra nor
the TSP mass spectra enabled differentiation of one position isomer from the other. Therefore a successful attempt was made
to distinguish the 6-C from the 8-C-glycosylflavones by thermospray tandem mass spectrometry (TSP-MS-MS). The collision induced dissociation (CID) spectra of
the particular ion M+H-120]+ gave fragments which permitted differentiation of position isomers. To confirm the accuracy of on-line results, reference
compounds were included in the HPLC study. |
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Keywords: | Column liquid chromatography mass spectrometry C-glycosylflavones Dissotis rotundifolia (Melastomataceae) Thermospray MS-MS |
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