| 分光光度法测定当归补血汤及其组分单味药提取液的抗氧化能力 |
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| 引用本文： | 孙悦,梁华中,詹晓丽.分光光度法测定当归补血汤及其组分单味药提取液的抗氧化能力[J].理化检验(化学分册),2010(3). |
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| 作者姓名： | 孙悦 梁华中 詹晓丽 |
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| 作者单位： | 广东药学院中药学院; |
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| 基金项目： | 广东省自然科学基金博士启动项目(845102240100160);;广东药学院博士科研启动项目(43548021) |
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| 摘 要： | 对复方当归补血汤及其组成单味药即当归和黄芪的水提取液,以及两者的单味药的水提取液及乙醇提取液的抗氧化能力作了比较研究。将由Fenton反应产生的羟自由基在pH4.6乙酸盐缓冲溶液与水杨酸反应,生成紫红色的2,3-二羟基苯甲酸,其吸收峰在510nm波长处。当有任一抗氧化组分存在于此反应体系时,由于其与反应体系中的羟自由基的优先反应,使此体系在510nm处的吸光度下降。据此,对上述药物提取液的抗氧化能力进行了试验。所得结果表明:上述药物的提取液均呈良好的抗氧化能力(即清除羟自由基的能力),而复方当归补血汤中主成分药物的提取液(无论是水或乙醇的提取液)的抗氧化能力均高于单味药物的提取液。比较水提取液与乙醇提取液的抗氧化能力的结果表明,前者的效果更好。
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| 关 键 词： | 复方当归补血汤 当归 黄芪 羟自由基 分光光度法 |
Spectrophotometric Determination of Anti-oxidation Capacity of Extract of Dangguibuxuetang Decoction and the 2 Componental Single Medicinal Herbs |
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| SUN Yue,LIANG Hua-zhong,ZHAN Xiao-li.Spectrophotometric Determination of Anti-oxidation Capacity of Extract of Dangguibuxuetang Decoction and the 2 Componental Single Medicinal Herbs[J].Physical Testing and Chemical Analysis Part B:Chemical Analgsis,2010(3). |
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| Authors: | SUN Yue LIANG Hua-zhong ZHAN Xiao-li |
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| Institution: | Dept.of Traditional Chinese Medicine/a>;Guangdong College of Pharmacology/a>;Guangzhou 510006/a>;China |
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| Abstract: | A comparative study on the anti-oxidation capacity of aqueous and ethanol extracts containing angelica and astragalus membranaceus of the Dangguibuxuetang decoction and the 2 componental single herbs was made.Hydroxyl free radical produced by the Fenton reaction was reacted with salicylic acid in an acetate buffer medium of pH 4.6,to form a reddish violet product,2,3-dihydroxybenzoic acid with its absorption maximum at 510 nm,where the absorbance was measured.When any of the anti-oxidative ingredient in the... |
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| Keywords: | Angelica compound haematic decoction Angelica Astragalus membranaceus Hydroxyl free radical Spectrophotometry |
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