中红外光纤技术用于口腔肿瘤在体原位检测的研究

肿瘤是严重威胁人类健康和生命的疾病，早期诊断和及时治疗是提高肿瘤病人存活率的重要因素，肿瘤的发生和发展一般可分为3个阶段：(1)基因突变；(2)生物分子组成和结构发生改变；(3)细胞和组织形态发生变化，目前常用的影像学方法只能检测较大的肿块，组织标本的病理诊断法需在

In Vivo and Rapid Detection of the Malignancy Tumor of Oral Tissues Using Mid-FTIR Fiber Optics Spectroscopy

Our previous studies show that mid-FTIR spectroscopy can be used to distinguish malignant oral tissue from normal tissue under in vitro condition. Here, an in-situ FTIR spectroscopic measurement was performed to record FTIR spectra of normal and malignant oral tissues including salivary gland, tongue, parotid gland, submandibular gland etc. during clinical examination. The FTIR spectra of various oral tissues were acquired when an ATR probe linked to the FTIR spectrometer via mid-IR optical fibers was pressed on the tissues of the patients. For example, a patient(male, 76 years old) with tumor on the left parotid and the corresponding normal tissue on the right parotid were measured and obvious differences were observed. The spectral features of normal tissue and tumor are in good agreement with the criteria established in our previous work. (1) 1 389 cm -1 band is quite strong in tumor, while the corresponding band in normal tissue is weaker than 1 452 cm -1 band. (2) In normal tissue, 1 250 cm -1 band is stronger, but the 1 250 cm -1 band disappeared in the skin of malignant tissue. The above results demonstrate that in vivo FTIR spectra are in good agreement with our previous results obtained under in vitro condition. We believe that in vivo FTIR spectroscopy, providing the first-hand information concerning whether the suspected tissue is cancerous or not, is helpful for doctors in clinical activity.