Addition of p-azido-L-phenylalanine to the genetic code of Escherichia coli |
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| Authors: | Chin Jason W Santoro Stephen W Martin Andrew B King David S Wang Lei Schultz Peter G |
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| Institution: | Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA. |
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| Abstract: | We report the selection of a new orthogonal aminoacyl tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in response to the amber codon, TAG. The amino acid is incorporated in good yield with high fidelity and can be used to crosslink interacting proteins. |
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