On-line single droplet deposition for MALDI mass spectrometry |
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Authors: | Xia?Zhang Damien?A?Narcisse Email author" target="_blank">Kermit?K?MurrayEmail author |
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Institution: | Department of Chemistry, Louisiana State University, Baton Rouge 70803, USA. |
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Abstract: | A single droplet generator was coupled with a rotating ball inlet matrix-assisted laser desorption/ionization (MALDI) time of flight (TOF) mass spectrometer. Single droplets with 100 picoliter volume were ejected by a piezoelectric-actuated droplet generator and deposited onto a matrix-coated rotating stainless steel ball at atmospheric pressure. The single droplet deposit was transported to the vacuum side of the instrument where ionization was accomplished using a UV pulsed laser. Using this on-line interface, it was possible to obtain protonated molecule signal from as little as 10 fmol analyte. |
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