Effect of media composition and growth conditions on production of β-glucosidase by Aspergillus niger C-6 |
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Authors: | O García-Kirchner M Segura-Granados P Rodríguez-Pascual |
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Institution: | (1) Departamentos de Bioprocesos y Biología de la Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional (UPIBI/IPN), Av. Acueducto s/n. Col. Barrio La Laguna, Ticomán, 07340 México, D. F. México |
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Abstract: | The hydrolytic activity of fungal originated β-glucosidase is exploited in several biotechnological processes to increase
the rate and extent of saccharification of several cellulosic materials by hydrolyzing the cellobiose which inhibits cellulases.
In a previous presentation, we reported the screening and liquid fermentation with Aspergillus niger, strain C-6 for β-glucosidase production at shake flask cultures in a basal culture medium with mineral salts, corn syrup liquor, and
different waste lignocellulosic materials as the sole carbon source obtaining the maximum enzymatic activity after 5–6 d of
8.5 IU/mL using native sugar cane bagasse. In this work we describe the evaluation of fermentation conditions: growth temperature,
medium composition, and pH, also the agitation and aeration effects for β-glucosidase production under submerged culture using
a culture media with corn syrup liquor (CSL) and native sugar cane bagasse pith as the sole carbon source in a laboratory
fermenter. The maximum enzyme titer of 7.2 IU/mL was obtained within 3 d of fermentation. This indicates that β-glucosidase
productivity by Aspergillus niger
C-6 is function of culture conditions, principally temperature, pH, culture medium conditions, and the oxygen supply given in
the bioreactor. Results obtained suggest that this strain is a potential microorganism that can reach a major level of enzyme
production and also for enzyme characterization. |
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Keywords: | Sugar cane bagasse pith Aspergillus niger β -glucosidase corn syrup liquor oxygen supply |
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