Optimization and Validation of an HPLC-UV Method for Determination of Tranexamic Acid in a Dosage Form and in Human Urine |
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Authors: | Ghada M Hadad Alaa El-Gindy Waleed M M Mahmoud |
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Institution: | (1) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, 41522, Egypt |
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Abstract: | A simple and reliable high-performance liquid chromatographic method with UV detection at 245 nm has been developed and validated
for determination of tranexamic acid (TA) in a dosage form and in human urine. Before injection samples were derivatized with
phenyl isothiocyanate (PITC). The reaction temperature, reaction time, and concentration of PITC used for derivatization were
optimized. Chromatographic separation was on a C18 column with a 65:35 (v/v) mixture of 10 mM phosphate buffer, pH 3.6, and acetonitrile as mobile phase. Heptaminol hydrochloride was used as internal
standard. |
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Keywords: | Column liquid chromatography Urinary excretion Tranexamic acid Phenyl isothiocyanate |
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