提高血性胸、腹水阳性检出率的制片法

取200例新鲜血性胸、腹水5～10mL倒入试管中,离心15～30min后吸弃上清液,分三段取沉淀物:①上清液与沉淀物之间的灰白色部分,②沉淀物中间部分,③沉淀物底部细胞;分别制片稍干后放入95%的乙醇固定液固定30min,HE染色.采用10×40倍显微镜涂片找异常细胞并对比分析.结果表明:上清液与沉淀物之间灰白色部分的细胞涂片见较多量癌肿细胞,癌肿细胞的占总病例43%,远大于其它二段沉淀物的阳性率,具有显著性统计学差异(P<0.01).采用分段取沉淀物法简便、有效,可提高血性胸、腹水癌肿细胞阳性率.

Improving positive production method of bloody-pleural effusion

The author taking the fresh bloody-pleural effusion 5～10 mL from 200 cases into the test tube, 15~ 30 min after the suction centrifugal supernatant discarded, three operative paragraphs, taking sediment: ①The gray part between the supernatant and sediment, ②The middle part of the sediment, ③The bottom cell of sediment; Slightly dry all parts were film into 95% ethanol fixative fixed-30min, HE staining. Smear observed using 10x40 microscopy to find abnormal cells are compared and analysed.The test result: Th...