A design of fluorescent probes for superoxide based on a nonredox mechanism |
| |
Authors: | Maeda Hatsuo Yamamoto Kayoko Nomura Yoko Kohno Iho Hafsi Leila Ueda Noritsugu Yoshida Shoko Fukuda Masako Fukuyasu Yuka Yamauchi Yuji Itoh Norio |
| |
Institution: | Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, Osaka 565-0871, Japan. h-maeda@phs.osaka-u.ac.jp |
| |
Abstract: | Fluorometric detection of O2-* is performed based on desulfonylation of 3 to the corresponding fluoresceins 4 through nucleophilic substitution, and this fluorescing process is quite specific toward O2-* over H2O2, t-BuOOH, NaOCl, 1O2, HO*, NO*, and ONOO-. Furthermore, effects of glutathione, cytochrome P450 reductase/NADPH, and diaphorase/NADH are relatively small on the fluorescing process of probe 3 with X = Y = F, which is useful to detect O2-* released from neutrophils stimulated by phorbol myristate acetate with satisfactory sensitivity. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|