Oxidative stress and immunologic responses following a dietary exposure to PAHs in <Emphasis Type="Italic">Mya arenaria</Emphasis> |
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Authors: | Nicolas Pichaud Jocelyne Pellerin Michel Fournier Sophie Gauthier-Clerc Pascal Rioux Émilien Pelletier |
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Institution: | 1.Institut des Sciences de la Mer de Rimouski,Rimouski,Canada;2.INRS- Institut Armand-Frappier,Laval,Canada |
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Abstract: | Background The aim of this research was to investigate oxidative stress and immune responses following a dietary polycyclic aromatic
hydrocarbon (PAH) exposure in a marine bioindicator organism, the soft shell clam, Mya arenaria. Immune parameters in hemolymph (haemocyte number, efficiency of phagocytosis and haemocyte activity) and assessment of oxidative
stress using catalase (CAT) activity and levels of malondialdehyde (MDA) performed on the digestive gland were estimated as
biomarkers in clams fed in mesocosm with PAH contaminated phytoplankton. MDA levels and CAT activities were also measured
in situ in organisms sampled in a control site (Metis Beach, Québec, Canada) as well as organisms sampled in a site receiving domestic
effluents (Pointe-au-Père, Québec, Canada), to assess effects of abiotic variables related to seasonal variations and mixed
contamination on the selected parameters. |
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