Effects of hydroxyapatite nanoparticles on proliferation and apoptosis of human breast cancer cells (MCF-7) |
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Authors: | Ramovatar Meena Kavindra Kumar Kesari Madhu Rani R Paulraj |
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Institution: | (1) School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, 110067, India;(2) School of Biotechnology, Jawaharlal Nehru University, New Delhi, 110067, India; |
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Abstract: | The study aimed to correlate cell proliferation inhibition with oxidative stress and p53 protein expression in cancerous cells.
Hydroxyapatite (HAP) (Ca10(PO4)6(OH)2) is the essential component of inorganic composition in human bone. It has been found to have obvious inhibitory function
on growth of many kinds of tumor cells and its nanoparticle has stronger anti-cancerous effect than macromolecule microparticles.
Human breast cancer cells (MCF-7) were cultured and treated with HAP nanoparticles at various concentrations. Cells viability
was detected with MTT colorimetric assay. The morphology of the cancerous cells was performed by transmission electron microscopy
and the expression of a cell apoptosis related gene (p53) was determined by ELISA assay and flow cytometry (FCM). The intracellular
reactive oxygen species (ROS) level in HAP exposed cells was measured by H2DCFDA staining. DNA damage was measured by single-cell gel electrophoresis assay. The statistical analysis was done by one
way ANOVA. The cellular proliferation inhibition rate was significantly (p < 0.05) increasing in a dose-dependent manner of HAP nanoparticles. Cell apoptotic characters were observed after MCF-7 cells
were treated by HAP nanoparticles for 48 h. Moreover, ELISA assay and FCM shows a dose-dependent activation of p53 in MCF-7
cells treated with nanoHAP. These causative factors of the above results may be justified by an overproduction of ROS. In
this study, a significant (p < 0.05) increase in the level of intracellular ROS in HAP-treated cells was observed. This study shows that HAP inhibits
the growth of human breast cancer MCF-7 cells as well as induces cell apoptosis. This study shows that HAP NPs Induce the
production of intracellular reactive oxygen species and activate p53, which may be responsible for DNA damage and cell apoptosis. |
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