| 反相高效液相法测定苁蓉酒中松果菊苷的含量 |
| |
| 引用本文： | 雷丽,屠鹏飞,张建国,翟彦奎,余世臣. 反相高效液相法测定苁蓉酒中松果菊苷的含量[J]. 色谱, 2002, 20(6): 582-584 |
| |
| 作者姓名： | 雷丽 屠鹏飞 张建国 翟彦奎 余世臣 |
| |
| 作者单位： | 1. 北京大学药学院,中医药现代研究中心,北京,100083
2. 内蒙古阿拉善苁蓉集团公司,内蒙古,阿拉善左旗,750306 |
| |
| 摘 要： | 采用反相高效液相法测定苁蓉酒中松果菊苷的含量。该法能有效地排除复方中其他组分的干扰,使峰得到完全分离。条件:固定相为C18;流动相为乙腈 1%的乙酸水溶液(体积比为14∶86);柱温25℃;紫外检测波长为330nm。结果表明,在0 52μg～2 60μg内松果菊苷的进样量与其峰面积呈良好的线性关系;样品的加标回收率为99 7%。该方法快速、简便、结果稳定,重现性好,是一种有效的质量检验方法。
|
| 关 键 词： | 反相高效液相法 松果菊苷 苁蓉酒 |
| 文章编号： | 1000-8713(2002)06-0582-03 |
| 修稿时间： | 2002-03-05 |
Determination of Echinacoside in Congrong Spirit by Reversed-Phase High Performance Liquid Chromatography |
| |
| Li Lei,Peng-fei Tu,Jian-guo Zhang,Yan-kui Zhai,Shi-chen Yu. Determination of Echinacoside in Congrong Spirit by Reversed-Phase High Performance Liquid Chromatography[J]. Chinese journal of chromatography, 2002, 20(6): 582-584 |
| |
| Authors: | Li Lei Peng-fei Tu Jian-guo Zhang Yan-kui Zhai Shi-chen Yu |
| |
| Affiliation: | School of Pharmaceutical Sciences, Modern Research Center of Chinese Traditional Medicines, Peking University, Beijing 100083, China. |
| |
| Abstract: | A reversed-phase high performance liquid chromatographic method, which is relatively simple, rapid and effective for echinacoside determination in Congrong spirit, has been developed. With this method, the peak of echinacoside could be separated completely from other interfering components in the spirit. The chromatographic conditions used were a reversed-phase C18 column at 25 degrees C with detection wavelength at 330 nm and CH3CN-1% HAc (14:86 in volume ratio) as mobile phase. A good linear relationship has been obtained between 0.52 microgram to 2.60 micrograms of echinacoside. The recovery was 99.7%. |
| |
| Keywords: | reversed-phase high performance liquid chromatography echinacoside Congrong spirit |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《色谱》浏览原始摘要信息 |
|
点击此处可从《色谱》下载全文 |
|
