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THE KERATIN INTERMEDIATE FILAMENTLIKE SYSTEM IN THE PLANT MESOPHYLL CELLS
引用本文:苏菲,顾伟,翟中和. THE KERATIN INTERMEDIATE FILAMENTLIKE SYSTEM IN THE PLANT MESOPHYLL CELLS[J]. 中国科学B辑(英文版), 1990, 0(9)
作者姓名:苏菲  顾伟  翟中和
作者单位:Department of Biology,Peking University,Beijing 100871,PRC,Department of Biology,Peking University,Beijing 100871,PRC,Department of Biology,Peking University,Beijing 100871,PRC
摘    要:A delicate intermediate filament-like network of mesophyll cells was observed both in maize and tobacco, using selective extraction together with whole-mount cell preparation for electron microscopy. The filament of the network is about 10 nm in diameter. Further test using immuno-gold labeling with anti-keratin antibodies indicated that the component of the intermediate filament-like system was keratin-like protein. Such a keratin-like intermediate filament system existing in plant cells was demonstrated for the first time. Meanwhile, 3-nm size filaments and their connection with 10-nm filaments were also shown in maize and tobacco protoplasts.


THE KERATIN INTERMEDIATE FILAMENTLIKE SYSTEM IN THE PLANT MESOPHYLL CELLS
SU FEI GU WEI ZHAI ZHONG-HE. THE KERATIN INTERMEDIATE FILAMENTLIKE SYSTEM IN THE PLANT MESOPHYLL CELLS[J]. Science in China(Chemistry), 1990, 0(9)
Authors:SU FEI GU WEI ZHAI ZHONG-HE
Abstract:A delicate intermediate filament-like network of mesophyll cells was observed both in maize and tobacco, using selective extraction together with whole-mount cell preparation for electron microscopy. The filament of the network is about 10 nm in diameter. Further test using immuno-gold labeling with anti-keratin antibodies indicated that the component of the intermediate filament-like system was keratin-like protein. Such a keratin-like intermediate filament system existing in plant cells was demonstrated for the first time. Meanwhile, 3-nm size filaments and their connection with 10-nm filaments were also shown in maize and tobacco protoplasts.
Keywords:intermediate filaments  plant mesophyll cells  keratin  immuno-gold electron microscopy
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