Enzymatic transformation of cephalosporin C to 7-ACA by simultaneous action of immobilized d-amino acid oxidase and glutaryl-7-ACA acylase |
| |
Authors: | Daniele Bianchi Rossella Bortolo Paolo Golini Pietro Cesti |
| |
Institution: | (1) EniChem S.p.A.-Centro Ricerche Novara, Istituto G. Donegani, Via Fauser 4, 1-28100 Novara, Italy |
| |
Abstract: | The enzymatic transformation of cephalosporin C (CEPH C) to 7-amino-cephalosporanic acid (7-ACA) using D-amino acid oxidase
(DAO) and glutaryl-7-ACA acylase (G1-7-ACA) is reported. The enzymes have been immobilized separately on different carriers,
in order to maximize the catalytic activity and the stability. The reaction has been carried out in single-step-like conditions,
using the two enzymes simultaneously. The effect of catalase contamination, present in the DAO preparations, was balanced
by addition of extra hydrogen peroxide. In optimum conditions, the conversion of CEPH C to 7-ACA was higher than 90%, with
byproduct formation lower than 4%. The mixture of immobilized enzymes was reused in repeated reaction cycles, showing an appreciable
operational stability. |
| |
Keywords: | Enzymatic transformation cephalosporin C 7-aminocephalosporanic acid D-amino acid oxidase glutaryl-7-ACA acylase |
本文献已被 SpringerLink 等数据库收录! |
|