| 光谱法研究硫鸟嘌呤与七元瓜环及牛血清白蛋白的超分子相互作用 |
| |
| 引用本文： | 黄英,王娟,郭改英,陶朱,薛赛凤,祝黔江,周清娣. 光谱法研究硫鸟嘌呤与七元瓜环及牛血清白蛋白的超分子相互作用[J]. 高等学校化学学报, 2013, 34(2): 375-380. DOI: 10.7503/cjcu20120645 |
| |
| 作者姓名： | 黄英 王娟 郭改英 陶朱 薛赛凤 祝黔江 周清娣 |
| |
| 作者单位： | 1. 贵州大学西南药用生物资源教育部工程研究中心;2. 贵州省大环化学及超分子化学重点实验室, 贵阳 550025;3. 悉尼大学化学院, 澳大利亚新南威尔士州 2006 |
| |
| 基金项目： | 国家自然科学基金(批准号:20972034);贵州省教育厅自然科学基金(批准号:黔教科2010013号);贵州省科技厅国际合作项目(批准号:黔科合外G字[2012]7003号);教育部春晖计划项目(批准号:Z2011034)资助 |
| |
| 摘 要： | 利用紫外-可见吸收光谱法和荧光光谱法研究了抗癌药物硫鸟嘌呤(6-TG)与七元瓜环(Q[7])及牛血清白蛋白(BSA)的相互作用. 结果表明, 6-TG与Q[7]及BSA可形成三元复合物, 且6-TG与Q[7]及BSA均可形成1:1的超分子配合物, 6-TG能引起BSA的荧光猝灭, 猝灭机制为静态猝灭. 此外, 还用同步荧光法和三维荧光法考察了6-TG对BSA构象的影响, 结果表明6-TG的加入使BSA的构象发生了变化, 而同步荧光光谱结果表明结合位点更接近于色氨酸.
|
| 关 键 词： | 硫鸟嘌呤 七元瓜环 牛血清白蛋白 紫外-可见吸收光谱 荧光光谱 |
| 收稿时间： | 2012-07-06 |
Interaction of 6-Thioguanine with Cucurbit[7]uril and Bovine Serum Albumin by Spectroscopic Method |
| |
| HUANG Ying,WANG Juan,GUO Gai-Ying,TAO Zhu,XUE Sai-Feng,ZHU Qian-Jiang,ZHOU Qing-Di. Interaction of 6-Thioguanine with Cucurbit[7]uril and Bovine Serum Albumin by Spectroscopic Method[J]. Chemical Research In Chinese Universities, 2013, 34(2): 375-380. DOI: 10.7503/cjcu20120645 |
| |
| Authors: | HUANG Ying WANG Juan GUO Gai-Ying TAO Zhu XUE Sai-Feng ZHU Qian-Jiang ZHOU Qing-Di |
| |
| Affiliation: | 1. The Engineering and Research Center for Southwest Bio-Pharmaceutical Resources, Ministry of Education;2. Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province, Guizhou University, Guiyang 550025, China;3. School of Chemistry, the University of Sydney, NSW 2006, Australia |
| |
| Abstract: | The aim of the present study was to investigate the interaction of 6-thioguanine(6-TG) with cucurbit[7]uril(Q[7]) and bovine serum albumin(BSA) in an acetate buffer solution by ultraviolet absorption spectroscopy, fluorescence emission spectroscopy and synchronous fluorescence spectra, as well as and three-dimensional fluorescence spectra. It was indicated from UV-Vis spectra that 6-TG-Q[7] binary complex formed. The fluorescence quenching of BSA by 6-TG was a result of the formation of 6-TG-BSA complex, and the quenching mechanism of BSA by 6-TG was static quenching mechanism. Moreover, effect of 6-TG on BSA conformation was discussed by the studies of synchronous and three-dimensional fluorescence spectra. In addition, a specific binding interaction took place between the 6-TG and Q[7] in the presence of BSA. |
| |
| Keywords: | 6-Thioguanine Cucurbit[7]uril Bovine serum albumin Ultraviolet absorption spectroscopy Fluorescence spectroscopy |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《高等学校化学学报》浏览原始摘要信息 |
|
点击此处可从《高等学校化学学报》下载全文 |
|
