| 高效液相色谱法测定动物组织中鱼腥草素同系物含量 |
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| 引用本文： | 李逐波,叶小利,袁吕江,李学刚. 高效液相色谱法测定动物组织中鱼腥草素同系物含量[J]. 分析化学, 2007, 35(2): 297-300 |
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| 作者姓名： | 李逐波 叶小利 袁吕江 李学刚 |
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| 作者单位： | 西南大学药学院药用资源化学研究所,重庆,400715;西南大学生命科学学院,重庆,400715 |
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| 基金项目： | 国家自然科学基金资助项目(No.20673084) |
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| 摘 要： | 以氢氧化钠水解合成鱼腥草素类药物,然后用乙醚提取,除去乙醚后,用HPLC法测定大鼠血样和组织样品中药物的含量。结果表明:碱水解后合成鱼腥草素,形成共轭双键的烯醇式烷酰乙醛,紫外吸收显著增强,HPLC图谱测定物峰分离良好。血液加样标准曲线回归线性良好,r=0.999以上,线性范围为0.1-100mg/L,标准加入回收率为97.3%。利用该方法分析合成鱼腥草素类药物在动物血液、脑、肝、心脏、肾脏和肌肉等组织中的残留完全可行。
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| 关 键 词： | 鱼腥草素同系物 水解 高效液相色谱 组织残留 |
| 修稿时间： | 2006-04-172006-10-13 |
Analysis of the Content of Houttuyfonate Homologues in Tissues of Animal by High Performance Liquid Chromatography |
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| Li Zhu-Bo,Ye Xiao-Li,Yuan Lü-Jiang,Li Xue-Gang. Analysis of the Content of Houttuyfonate Homologues in Tissues of Animal by High Performance Liquid Chromatography[J]. Chinese Journal of Analytical Chemistry, 2007, 35(2): 297-300 |
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| Authors: | Li Zhu-Bo Ye Xiao-Li Yuan Lü-Jiang Li Xue-Gang |
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| Affiliation: | 1.Chemistry Institute of Pharma, ceutical Resources, College of Pharmaceutical Science, Southwest University, Chongqing 400715;2.School of Life Science, Southwest University, Chongqing 400716 |
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| Abstract: | Houttuyfonate homologues(HOU)-Cn was hydrolyzed with NaOH and extracted by ether.After remov ether,the contents of HOU-Cn in blood and tissue of rat were determined by HPLC.The results show that hydrolyzed HOU-Cnwith NaOH would form enol alkyl aldehyde with conjugated double bonds,which makes the absorbance of HOU-Cn at 285 nm strengthened and the effect of isolation by HPLC better.The recovery rate by standard addition was about 97.3%,and the linearity range was 0.1 to 100 mg/L(r>0.999).It indicated that the method by hydrolyzing HOU-Cn with NaOH was feasible to analyze the content of HOU-Cn in some body liquid and tissues of animal,such as blood,brain,liver,heart,kidney and muscle. |
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| Keywords: | Houttuyfonate homologues hydrolysis high performance liquid chromatography residue in tissues |
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