PHOTOCHEMISTRY OF HEPARIN-ACRIDINE ORANGE COMPLEXES IN SOLUTION: PHOTOCHEMICAL CHANGES OCCURRING IN THE DYE AND POLYMER ON FLUORESCENCE FADING |
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| Authors: | J M Menter R E Hurst S S West |
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| Institution: | Department of Engineering Biophysics, University of Alabama in Birmingham. University Station, Birmingham, AL 35294., U.S.A. |
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| Abstract: | Abstract— Photolysis of acridine-orange-heparin complexes in N2- and O2-saturated solution results in permanent photooxidation of AO with little or no concomitant change in the heparin moiety. The major photoproduct is mono- N -demethylated acridine orange; in O2-saturated solution an additional minor oxygenated product, most likely the 10-oxide (N-oxide) or the 9-acridanone (acridone) is also formed. The results suggest an intermolecular electron transfer between adjacently adsorbed dye molecules. The heparin moiety plays a significant role in the photochemistry by bringing dye molecules into favorable geometric orientation for biomolecular reaction and by means of specific dye-polymer interactions. |
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