Expression of Trichoderma reesei exo-cellobiohydrolase I in transgenic tobacco leaves and calli |
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Authors: | Ziyu Dai Brian S. Hooker Ryan D. Quesenberry Jianwei Gao |
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Affiliation: | (1) Bioprocessing Group, Environmental Technological Division, Pacific Northwest National Laboratory, MSIN K2-10, P.B. Box 999 Richland, WA, 99352 |
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Abstract: | Expression of Trichoderma reesei exo-cellobiohydrolase I (CBHI) gene in transgenic tobacco was under the control of CaMV 35S promoter. In transgenic leaf tissues, CBHI activity up to 66.1 μmol/h/g total protein was observed. In transgenic calli, the highest CBHI activity was 83.6 μmol h/g total protein. Protein immunoblot analysis confirms the presence of CBHI enzyme in both transgenic calli and leaf tissues. CBHI expression levels accounted for about 0.11% and 0.082% of total protein in transgenic leaf tissues and calli, respectively, Furthermore, expression of CBHI gene did not affect normal growth and development of transgenic plants. |
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Keywords: | Bioreactor cellulase exo-cellobiohydrolase I transgenic tobacco Trichoderma reesei |
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