A study of noncovalent protein complexes by matrix-assisted laser desorption/ionization |
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| Authors: | Fenhong Song |
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| Institution: | Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland, USA. fenhong.song@fda.hhs.gov |
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| Abstract: | A sample preparation method has been developed for detection of noncovalent protein-protein complexes by MALDI in this work. An aqueous solution of the matrix at pH 7 allows the reproducible detection of a protein dimer, a protein tetramer, and a heterodimer. The signals are stable under long irradiation and can be detected at wide ranges of concentrations and with different laser intensities. |
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