Detection of polar and macrocyclic trichothecene mycotoxins from indoor environments |
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Authors: | Tuomi T Saarinen L Reijula K |
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Institution: | Finnish Institute of Occupational Health, Uusimaa Regional Institute, Helsinki, Finland. tapani.tuomi@occuphealth.fi |
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Abstract: | A method is described for the qualitative and semi-quantitative simultaneous determination of both non-macrocyclic and macrocyclic trichothecene biotoxins from samples derived from indoor environments. The method includes extraction, sample pre-treatment and reversed-phase HPLC separation followed by tandem mass spectrometric identification and quantification using electrospray ionization on a quadrupole ion trap mass analyser. Aqueous methanol was used in the initial extraction and solvent partitioning and solid-phase extraction in the purification of samples. The HPLC separation was run on-line with electrospray ionization MS-MS detection. The detection limits and recoveries of the procedure varied from 1 to 1000 pg and from 31 to 92%, respectively. As the method includes few and not very labour intensive sample treatment steps, it should allow for a high throughput of samples with good prospects of automation. |
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