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MISTRAL: a transmission soft X‐ray microscopy beamline for cryo nano‐tomography of biological samples and magnetic domains imaging
Authors:Andrea Sorrentino  Josep Nicolás  Ricardo Valcárcel  Francisco Javier Chichón  Marc Rosanes  Jose Avila  Andrei Tkachuk  Jeff Irwin  Salvador Ferrer  Eva Pereiro
Abstract:The performance of MISTRAL is reported, the soft X‐ray transmission microscopy beamline at the ALBA light source (Barcelona, Spain) which is primarily dedicated to cryo soft X‐ray tomography (cryo‐SXT) for three‐dimensional visualization of whole unstained cells at spatial resolutions down to 30 nm (half pitch). Short acquisition times allowing for high‐throughput and correlative microscopy studies have promoted cryo‐SXT as an emerging cellular imaging tool for structural cell biologists bridging the gap between optical and electron microscopy. In addition, the beamline offers the possibility of imaging magnetic domains in thin magnetic films that are illustrated here with an example.
Keywords:X‐ray microscopy  tomography  correlative imaging  magnetic domains imaging
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