MODIFICATION OF α-CHYMOTRYPSIN USING A WATER-SOLUBLE PHOTO-FENTON REAGENT |
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| Authors: | Hiromi Kitano Yasushi Maeda Kanae Furukawa Takahiro Yamamoto Rie Izumida Seiichi Matsugo |
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| Institution: | Department of Chemicai and Biochemical Engineering, Toyama University, Toyama, 930 Japan |
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| Abstract: | Abstract— -Modification of an enzyme, α-chymotrypsin, was examined by using a water-soluble photo-Fenton reagent. By photoirradiation of the enzyme with the reagent, which can occupy a binding site of the enzyme, a tryptophan residue in the vicinity of the active site was oxidized to N -formylkynurenine. Concurrently, the catalytic properties of the enzyme were largely changed: the K m was increased and the k catwas decreased. The decrease in k cat for a specific amide substrate was the most significant among the esters and amides examined. The water-soluble photo-Fenton reagent would be useful to chemically modify relatively limited regions in biomolecules. |
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