Construction and Cleavage Characterization of Single Targeted Multi-ribozyme Expression Systems in vitro |
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| Authors: | TIAN Sheng-Li XU Dong-ping OHNUMA Ta-kao |
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| Institution: | [1]Shenzhen Key Laboratory of Microbiology and Gene Engineering, College of Life Sciences, Shenzhen University, Shenzhen 518060, P. R. China [2]The 302 Hospital of Beijing, Beijing 100039, P R. China [3]Mount Sinai School of Medicine, New York 10029, USA |
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| Abstract: | In order to clarify cleavage efficiency of a multi-ribozyme expression system on their substrates,plasmids containing 20 cis-acting hammerhead ribozymes for self-cleavage and 10 trans-acting hammerhead ribozymes targeted on MDR1 and MRP1 substrates,the target plasmids pGEM-MDR1 and pGEM-MRP1 were constructed by employing PCR and DNA recombination.The endonuclease's digestion and DNA sequencing confirmed exactness of the multi-ribozyme expression systems and the target plasmids.The repeated self-cleavage tes... |
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| Keywords: | Multi-ribozyme expression systems Hammerhead ribozyme RNA substrate |
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