| 替米考星分子印迹聚合物的制备及其固相萃取研究 |
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| 引用本文： | 郑亚秋,曹湛,郭宏斌,张青杰,贺利民,陈清菊,骆飚.替米考星分子印迹聚合物的制备及其固相萃取研究[J].分析化学,2010,38(1). |
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| 作者姓名： | 郑亚秋 曹湛 郭宏斌 张青杰 贺利民 陈清菊 骆飚 |
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| 作者单位： | 华南农业大学兽医学院药理研究室,广州,510642 |
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| 基金项目： | 农业行业标准资金(No.200103151)资助 |
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| 摘 要： | 以泰乐菌素为虚拟模板分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,合成了对替米考星具有高选择性的分子印迹聚合物。考察了二甲基甲酰胺、甲醇、丙酮和氯仿4种致孔剂对合成聚合物性能的影响。通过正交实验优化的聚合配方为:1.0mmol泰乐菌素,8.0mmol甲基丙烯酸,20.0mmol乙二醇二甲基丙烯酸酯,6.0mL氯仿,20.0mg偶氮二异丁腈。研究了将该聚合物作为固相萃取填料分离、富集替米考星的萃取条件和萃取性能。当萃取柱依次用甲醇和水(pH9.0)活化,乙腈溶液上样,甲醇和氯仿分别洗涤,3mL氨化甲醇(95:5,V/V)洗脱时,替米考星在分子印迹固相萃取柱上的回收率达到90%以上,而非分子印迹固相萃取柱的回收率仅为32%。
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| 关 键 词： | 分子印迹聚合物 替米考星 固相萃取 虚拟模板 |
Preparation of Tilmicosin Imprinted Polymers and Its Application to Solid Phase Extraction |
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| ZHENG Ya-Qiu,CAO Zhan,GUO Hong-Bin,ZHANG Qing-Jie,HE Li-Min,CHEN Qing-Ju,LUO Biao.Preparation of Tilmicosin Imprinted Polymers and Its Application to Solid Phase Extraction[J].Chinese Journal of Analytical Chemistry,2010,38(1). |
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| Authors: | ZHENG Ya-Qiu CAO Zhan GUO Hong-Bin ZHANG Qing-Jie HE Li-Min CHEN Qing-Ju LUO Biao |
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| Institution: | Department of Pharmacology;College of Veterinary Medicine;South China Agricultural University;Guangzhou 510642 |
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| Abstract: | Molecularly imprinted polymers(MIPs) with high selectivity to tilmicosin(TIM) were prepared using tylosin(TYL) as dummy template,methacrylic acid(MAA) as monomer,ethylene glycol dimethacrylate (EGDMA) as cross-linker.The effects of 4 porogens including dimethyl formamide,methanol,acetone,and chloroform on the recognition capability of MIPs were investigated.Orthogonal test was used to optimize the preparation of MIPs,and the optimal composition was as follows:1.0 mmol TYL,8.0 mmol MAA,20.0 mmol EGDMA,6.0 mL... |
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| Keywords: | Molecularly imprinted polymers Tilmicosin Solid-phase extraction Dummy template |
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