An improved cryogen for plunge freezing |
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Authors: | William F Tivol Ariane Briegel Grant J Jensen |
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Institution: | California Institute of Technology, Broad Center, MC 114-96, 1201 E. California Blvd., Pasadena, CA 91125, USA. |
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Abstract: | The use of an alkane mixture that remains liquid at 77 K to freeze specimens has advantages over the use of a pure alkane that is solid at 77 K. It was found that a mixture of methane and ethane did not give a cooling rate adequate to produce vitreous ice, but a mixture of propane and ethane did result in vitreous ice. Furthermore, the latter mixture produced less damage to specimens mounted on a very thin, fragile holey carbon substrate. |
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